Analysis of GluPro aminoacyl synthetase.

Analysis performed on 2024-09-30 10:31:42

Number of Proteins Analyzed

Initial Filtered Out Final Blast
Bacteria 93268 1075 92193 100183
Archaea 6414 164 6250 6196
Vertebrata 1557 316 1241 463
Mammals 545 81 464 131

Number of Variants Analyzed:

Protein Length Pathogenic Variants Benign/Unknown Variants
1512 15 1225

Domains

Domains
Current Study 1, 154 | 694, 1007
Guo et al (2010) 1, 178 | 749, 805 | 822, 878 | 900, 956

Results

Mutations in Ancient vs Modern Domains

Current Study

Ancient Domains Modern Domains All Ratio
Number of Residues 1045 467 1512 2.237687
Number of Variants 838 402 1240 2.084577
Number of Pathogenic Variants 11 4 15 2.750000
Number of Benign/Unknown Variants 827 398 1225 2.077889
Fisher exact test 0.784986

The Fisher exact test was calculated using a contingency table with the number of pathogenic and benign/unknown variants in ancient and modern domains. The bottom left values.

Guo et al (2010)

Ancient Domains Modern Domains All Ratio
Number of Residues 1163 349 1512 3.332378
Number of Variants 923 317 1240 2.911672
Number of Pathogenic Variants 11 4 15 2.750000
Number of Benign/Unknown Variants 912 313 1225 2.913738
Fisher exact test 1.000000

The Fisher exact test was calculated using a contingency table with the number of pathogenic and benign/unknown variants in ancient and modern domains. The bottom left values.

Coverage of the alignment and domain locations

Agreement between the current study and Guo et al (2010)

Conservation of the amino acids in modern and ancient domains

# of sites Ancient # sites Modern Mean Ancient (SD) Mean Modern (SD) Median Ancient Median Modern Mann-Whitney U p-value
Current Study 1045 467 0.79 (0.01) 0.70 (0.01) 8.233e-01 6.963e-01 311494.50 3.86e-18
Guo et al (2010) 1163 349 0.78 (0.01) 0.69 (0.01) 8.127e-01 6.865e-01 259965.50 7.87e-16

The Mann-Whitney U test was calculated using the conservation scores of the amino acids in ancient and modern domains. The p-value is for the null hypothesis that the conservation scores in ancient domains are greater than in modern domains.

Conservation of the amino acids in modern and ancient domains